Australian Grape & Wine Events Calendar


CSIRO Plant Industry Seminar “Understanding the regulation of VvDXS gene expression”

Date/Time
Date(s) - 18 Jun 2013
4:00 PM - 5:00 PM

Organiser
CSIRO

Cost
Free

Contact Details
Virginia Barber
Phone: 08 83038600
Email: [email protected]

Event Region(s)
Adelaide

Category(ies)


 
  WIC West Building (upstairs seminar room) Corner Hartley Grove & Paratoo Rd, Waite Campus Urrbrae 4 pm | Tuesday June 18th (followed by informal discussion, drink & nibbles)  
  Dr Juri Battilana Visiting ScientistCSIRO Plant Industry   “Understanding the regulation of VvDXS gene expression”   Some of the most relevant wine odor constituents are monoterpenoids which have been demonstrated to be produced via the plastid-located methyl-erythritol-phosphate (MEP) pathway in grapevine. The MEP pathway biosynthetic gene 1-deoxy-D-xylulose 5-phosphate synthase (VvDXS), maps to a major QTL responsible for monoterpenoid accumulation in Muscat grape varieties. Recent results suggest that gain-of-function mutations that affect the enzymatic or regulatory properties of the VvDXS protein appear to be the major determinants of terpenoid accumulation. The 5’upstream region of VvDXS alleles were cloned and sequenced to characterize the promoter and several cis-elements putatively involved in the regulation of the gene were identified. This information was then used to screen a transcription factor library using a transient-expression, dual-luciferase assay in tobacco. It is also known that genomic DNA can be compacted into a chromatin form which limits the accessibility of transcription factors trying to bind to DNA target elements. Antibodies that recognize specific histone amino acid methylation patterns that are associated with heterochromatin or euchromatin allow us to determine epigenetic profiles. Preliminary results suggest chromatin modification differs within the coding region or promoters of the VvDXS gene depending on grapevine variety and on the stage of berry development. Furthermore, two clones of Chardonnay that exhibit dramatic differences in monoterpenoid accumulation and berry flavor have been characterized. They represent an ideal comparison to identify rare alleles of genes controlling the biosynthesis of aromatic compounds.   Dr Juri Battilana is a post-doc researcher at the Genomics and Biology of Fruit Crop Department, Research and Innovation Centre – Fondazione Edmund Mach San Michele all’Adige, Italy. In 2008, Juri received his PhD at the University of Padua, Faculty of Mathematical, Physical and Natural Sciences, Doctoral school in Agronomy and Crop Science. In the course of his PhD work in the Grapevine Applied Genomics Research Group headed by Dr Stella Grando, he analysed transcriptomic and metabolic profiles of berry samples from aromatic and non-aromatic grapes during ripening. He was also involved in the functional characterization of candidate genes by enzymatic assay experiments. Recently, thanks to a fellowship cofounded by EU research framework program Marie Curie Actions and Provincia Autonoma di Trento, he has been working in collaboration with Dr Paul Boss on the metabolic and genetic regulation of the biosynthesis of flavors and aromas in grape.  
 

For information contact: Paul Boss | [email protected] | 08 83038614